molecular detection of infectious bronchitis and newcastle disease viruses in broiler chickens with respiratory signs using duplex rt-pcr

نویسندگان

aylar saba shirvan

karim mardani

چکیده

infectious bronchitis (ib) and newcastle disease (nd) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. in the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-rt-pcr. rna was extracted from vaccinal and reference strains of infectious bronchitis virus (ibv) and newcastle disease virus (ndv) and then cdna was synthesized. using two universal primer sets for detection of ibv and ndv, the duplex-rt-pcr was developed. in order to assess the efficiency of the developed duplex rt-pcr, a number of 12 broiler farms with the symptoms of respiratory tract infection was sampled (trachea, lung and kidney were sampled from affected birds suspicious for ibv and ndv infections). after rna extraction from tissues and cdna synthesis, the presence of ibv and ndv genome were investigated using duplex-pcr. the results showed that three of twelve examined broiler farms were positive for ibv and two farms were positive for ndv and ibv. the results revealed that the duplex-rt-pcr is a quick and sensitive procedure for simultaneously detecting ibv and ndv in birds with respiratory infections.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT-PCR

Infectious bronchitis (IB) and Newcastle disease (ND) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. In the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-RT-PCR. RNA was extracted...

متن کامل

Molecular detection of infectious bronchitis and Newcastle disease viruses in broiler chickens with respiratory signs using Duplex RT-PCR

Infectious bronchitis (IB) and Newcastle disease (ND) are highly contagious and the most economically important diseases of the poultry affecting respiratory tract and causing economic losses in poultry industry throughout the world. In the present study, the simultaneous detection and differentiation of causative agents of these diseases were investigated using duplex-RT-PCR. RNA was extracted...

متن کامل

Detection of Newcastle disease, H9N2 avian influenza, and infectious bronchitis viruses in respiratory diseases in backyard chickens in Ahvaz, Iran, in 2014-2015

Newcastle disease virus (NDV), avian influenza virus (AIV), and infectious bronchitis virus (IBV) are the most prevalent viral pathogens in the Iranian poultry industry. This study aimed to reveal the presence of these viruses in the backyard chickens in Ahvaz, located in the Southwest of Iran. A total of 100 chickens with respiratory signs and mortality were examined by taking the blood sample...

متن کامل

Synbiotic enhances immune responses against infectious bronchitis, infectious bursal disease, Newcastle disease and avian influenza in broiler chickens

Increased susceptibility of birds to avian pathogens in intensive husbandry system has emphasized on necessity of improvement of innate and specific immune responses of birds by the fast establishment of a beneficial microflora and immune stimulator factors to guarantee healthy and low-price products. During this study, 192 one-day-old broiler chicks (Ross-380) in four groups with three replica...

متن کامل

Detection of Avian Encephalomyelitis Virus in Broiler Chickens in Iran Using RT-PCR and Histopathological Methods

Avian encephalomyelitis is caused by a Tremovirus and primarily affects chickens. The virus can infect young chicks and cause nervous symptoms. Vaccines are used to control the disease in breeders. Recently, the occurrence of the disease is causing concern in the poultry industry. In the present study, we report a case of avian encephalomyelitis in one broiler farm in Kashan (center of Iran). T...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
veterinary research forum

ناشر: faculty of veterinary medicine, urmia university

ISSN 2008-8140

دوره 5

شماره 4 2014

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023